sequencing

jingels Fish123!

文件大小

du --block-size=1

track

track type=bigWig name="F1 sense" description="mRNA Expression of sense in F1" visibility=full autoScale=on color=0,0,255 yLineMark=3 yLineOnOff=on priority=10 bigDataUrl=ftp://tyche.uthsc.edu/user/xusheng/F1.negative.wig_all.bw
browser position chr2:136,539,186-136,608,164

wig2bw

1. generating bw file
/home/xusheng/mRNAseq_12samples/wigToBigWig B6.negative.wig_all.log_remove ../../bc_1/output/sam2wig_new2/mm9.chrom.sizes B6.negative.wig_all.bw &

2. track type=bigWig name="Example One" description="A bigWig file" bigDataUrl=http://genome.ucsc.edu/goldenPath/help/bigWigExample.bw

track type=wiggle_0 name="variableStep" description="variableStep format" \
visibility=full autoScale=off viewLimits=0.0:25.0 color=50,150,255 \
yLineMark=11.76 yLineOnOff=on priority=10

sge to pbs

service pbs_server restart
service maui restart

bigwigs

>>>
>>> Are you talking about the database contents of
>>> the wiggle data, or the source ascii files that
>>> are given to wigEncode ?
>>>
>>> If they are just the source ascii files, they
>>> can all go together with 'cat':
>>> $ cat files*.wig > result.wig
>>>
>>> If they have custom track and browser lines in them:
>>> $ egrep -v "^track|^browser" files*.wig > result.wig
>>>
>>> Or, all these can be sent into wigEncode:
>>> $ egrep -v "^track|^browser" files*.wig | wigEncode stdin result.wig
>>> result.wib
>>>
>>> We now try to distinguish source files with the suffix:
>>> .wigFixed or .wigVar
>>> for fixedStep or variableStep wiggle ascii data.
>>>
>>> You may also be interested in the more efficient
>>> encoding mechanism of wigToBigWig and the resulting .bw
>>> file which can be a URL resource. See also:
>>> http://genome.ucsc.edu/goldenPath/help/bigWig.html
>>> This encoder works for bedGraph file types too and is
>>> much more efficient:
>>> http://genome.ucsc.edu/goldenPath/help/bedgraph.html
>>>